Artwork from my daughter Lilly.
Red Blood Cell Staining in Zebrafish
“Primitive” red blood cells enter into circulation at ~ 24 hpf in zebrafish. Red blood cells are easily stained using the chemical compound “o-dianisidine”. Shown here are images of embryos stained at 48 hpf.
Abilene Research Symposium
The Holowiecki Lab will be presenting at the Abilene Research Symposium October 29th 2021. https://www.ttuhsc.edu/pharmacy/immunotherapeutics-biotechnology/symposium.aspx
Learning CRISPR-Cas Mutagenesis at Baylor
It has been a productive and informative week so far at Baylor Medical.
nkx 2.5 ISH
Shown here is the expression of nkx2.5 within a developing zebrafish heart at ~ 24 hpf. This gene is highly conserved and functions as a major transcriptional regulator of cardiac morphogenesis.
generating probes for in situ hybridization experiments
We have been busy this summer optimizing protocols. Shown below are images of fixed embryos between 20-24 hours post fertilization. The purple stain indicates specific locations of gene expression for gata1 (erythroid specific transcription factor) and vmhc (ventricular myosin heavy chain).
“linear heart tube” in situ staining for the gene myl7
UO biologists are using zebrafish to study COVID-19 effects
https://around.uoregon.edu/content/uo-biologists-are-using-zebrafish-study-covid-19-effects
Grants Focus on STEM Research at Christian Colleges
https://www.insidehighered.com/quicktakes/2021/05/21/grants-focus-stem-research-christian-colleges
Campus Recipients Announced to Receive Over $1.5 Million for STEM Research