Here you will find a list of techniques (and relevant information) that will be commonly used in the Holowiecki lab.<\/p>\n
Gene Expression Analysis<\/strong><\/p>\n \u00a0 \u00a0 \u00a0in situ<\/em> hybridization- detection of spacial and temporal gene expression patterns (using RNA probes)<\/p>\n qPCR-\u00a0 technique used to monitor the amplification of specific genes in “real-time”<\/p>\n fluorescent “in vivo<\/em>” monitoring of gene expression using stable transgenic lines<\/p>\n <\/p>\n Standard Molecular Techniques<\/strong><\/p>\n Although The Holowiecki lab uses many techniques that are specific to zebrafish, we also use techniques common to all molecular biology labs. For example, generation of\u00a0 the RNA probes required to perform in situ<\/em> hybridization may entail multiple techniques prior to actual in situ<\/em> analysis. These include: RNA isolation, cDNA synthesis, sub-cloning, restriction analysis and gel electrophoresis, primer design, DNA sequencing, and PCR.<\/p>\n","protected":false},"excerpt":{"rendered":" Here you will find a list of techniques (and relevant information) that will be commonly used in the Holowiecki lab. Gene Expression Analysis \u00a0 \u00a0 \u00a0in situ hybridization- detection of spacial and temporal gene expression patterns (using RNA probes) qPCR-\u00a0 technique used to monitor the amplification of specific genes in “real-time” fluorescent “in vivo” monitoring … Continue reading