{"id":223,"date":"2021-06-02T09:40:19","date_gmt":"2021-06-02T14:40:19","guid":{"rendered":"http:\/\/blogs.acu.edu\/holowieckilab\/?p=223"},"modified":"2021-06-02T09:46:46","modified_gmt":"2021-06-02T14:46:46","slug":"first-successful-in-situ-hybridization-in-the-holowiecki-lab","status":"publish","type":"post","link":"https:\/\/blogs.acu.edu\/holowieckilab\/2021\/06\/02\/first-successful-in-situ-hybridization-in-the-holowiecki-lab\/","title":{"rendered":"first successful in situ hybridization in the Holowiecki lab"},"content":{"rendered":"<p>Shown here is the typical work flow used to generate a &#8220;probe&#8221; used to detect gene expression in developing zebrafish embryos. This will be a commonly used technique in the Holowiecki lab to detect how exposure to environmental pollutants alters the expression of key genes involved in cardiac development.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-226\" src=\"http:\/\/blogs.acu.edu\/holowieckilab\/files\/2021\/06\/myl7-workflow-1-300x113.png\" alt=\"\" width=\"502\" height=\"189\" srcset=\"https:\/\/blogs.acu.edu\/holowieckilab\/files\/2021\/06\/myl7-workflow-1-300x113.png 300w, https:\/\/blogs.acu.edu\/holowieckilab\/files\/2021\/06\/myl7-workflow-1-490x184.png 490w, https:\/\/blogs.acu.edu\/holowieckilab\/files\/2021\/06\/myl7-workflow-1-150x56.png 150w, https:\/\/blogs.acu.edu\/holowieckilab\/files\/2021\/06\/myl7-workflow-1-768x289.png 768w, https:\/\/blogs.acu.edu\/holowieckilab\/files\/2021\/06\/myl7-workflow-1.png 1439w\" sizes=\"auto, (max-width: 502px) 100vw, 502px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Shown here is the typical work flow used to generate a &#8220;probe&#8221; used to detect gene expression in developing zebrafish embryos. This will be a commonly used technique in the Holowiecki lab to detect how exposure to environmental pollutants alters the expression of key genes involved in cardiac development.<\/p>\n","protected":false},"author":18394,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-223","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/posts\/223","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/users\/18394"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/comments?post=223"}],"version-history":[{"count":3,"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/posts\/223\/revisions"}],"predecessor-version":[{"id":228,"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/posts\/223\/revisions\/228"}],"wp:attachment":[{"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/media?parent=223"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/categories?post=223"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.acu.edu\/holowieckilab\/wp-json\/wp\/v2\/tags?post=223"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}